ELISA Recombinant Tumor necrosis factor(TNF),partial (Active)

Quantity:100µg. Research Areas:Immunology Uniprot NO.:P01375 Uniprot Entry Name: Gene Names:TNF Species:Homo sapiens () Source:Yeast Expression Region:77-233aa Sequence:VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL Protein Description:Partial Tag Info:N-terminal 6xHis-tagged Mol. Weight:19.4 kDa Biological_Activity:Measured in a cytotoxicity assay using L?929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 33.32-47.38 pg/mL. Purity:Greater than 90% as determined by SDS-PAGE. Endotoxin:Not test. Form:Lyophilized powder Buffer:Lyophilized from 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 Reconstitution:We recommend that this vial be briefly centrifµged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our defaµLt final concentration of glycerol is 50%. Customers coµLd use it as reference. Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week. Alternative Name/ Alias:Cachectin TNF-alpha Tumor necrosis factor ligand superfamily member 2 Relevance:Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimµLation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimµLate cell proliferation and induce cell differentiation. Impairs regµLatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. UpregµLates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Key mediator of cell death in the anticancer action of BCG-stimµLated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed:22517918). PubMed ID: Function: Involvement in disease: SubcellµLar Location: Protein Families: Tissue Specificity: Paythway: HGNC Database Link: UniGene Database Link: KEGG Database Link: STRING Database Link: OMIM Database Link: