ELISA 6*His Monoclonal anti-

Quantity :50µL Clone Number:3G5H8 Aliases:6*His, 6*His, His Tag, 6histag, 6XHis, HHHHHH, HIS, His Tag Product Type:Tag/Control Antibodies Immunogen Species: UniProt ID: Immunogen:HHHHHH(6x His) synthetic peptide conjµgate to KLH Raised in:Mouse Reactivity:All Tested Applications:ELISA, WB; Recommended dilution: WB:1:500-1:2000 Background:A His tag is a tag of 6 histidines added to the end of the protein that the DNA codes for, so the DNA with contain 6 repeats of the His codon. What it is used for is purifying the protein that the DNA codes for. Histidines are positively charged amino acids and a group of 6 of them allows the protein to bind to a negatively charged column. Histidines are also special in that they have a pKa around 7, meaning that once bound to the column they can easily be unbound. Clonality:Monoclonal Isotype:IgG2a Purification Method:>95%, Protein A purified Conjµgate:Non-conjµgated Buffer:Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 Form:Liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:6*His Research Areas: