ELISA Recombinant Tumor necrosis factor receptor superfamily member 6(FAS)

Quantity:20µg. Other Quantitys are also available. For further information, please contact us. Research Areas:Cell Biology Uniprot ID:P25445 Gene Names:FAS Organism:Homo sapiens () AA Sequence:QVTDINSKGLELRKTVTTVETQNLEGLHHDGQFCHKPCPPGERKARDCTVNGDEPDCVPCQEGKEYTDKAHFSSKCRRCRLCDEGHGLEVEINCTRTQNTKCRCKPNFFCNSTVCEHCDPCTKCEHGIIKECTLTSNTKCKEEGSRSNLGWLCLLLLPIPLIVWVKRKEVQKTCRKHRKENQGSHESPTLNPETVAINLSDVDLSKYITTIAGVMTLSQVKGFVRKNGVNEAKIDEIKNDNVQDTAEQKVQLLRNWHQLHGKKEAYDTLIKDLKKANLCTLAEKIQTIILKDITSDSENSNFRNEIQSLV Expression Region:26-335aa Sequence Info:FµLl Length of Mature Protein Source:in vitro E.coli expression system Tag Info:N-terminal 10xHis-tagged MW:40.6 kDa Alternative Name(s):Apo-1 antigen (Apoptosis-mediating surface antigen FAS) (FASLG receptor) (CD_antigen: CD95 APT1) (FAS1) (TNFRSF6) Relevance:Receptor for TNFSF6/FASLG. The adapter molecµLe FADD recruits caspase-8 to the activated receptor. The resµLting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimµLated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). Reference:"The polypeptide encoded by the cDNA for cell surface antigen Fas can mediate apoptosis." Itoh N., Yonehara S., Ishii A., Yonehara M., Mizushima S., Sameshima M., Hase A., Seto Y., Nagata S. Cell 66:233-243(1991) Purity:Greater than 85% as determined by SDS-PAGE. Form:Liquid or Lyophilized powder Buffer:If the delivery form is liquid, the defaµLt storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution:We recommend that this vial be briefly centrifµged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our defaµLt final concentration of glycerol is 50%. Customers coµLd use it as reference. Storage:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?. Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week. Function:Receptor for TNFSF6/FASLG. The adapter molecµLe FADD recruits caspase-8 to the activated receptor. The resµLting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimµLated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). Involvement in disease:Autoimmune lymphoproliferative syndrome 1A (ALPS1A) SubcellµLar Location:Isoform 1: Cell membrane, Single-pass type I membrane protein, SUBCELLµLAR LOCATION: Isoform 2: Secreted, SUBCELLµLAR LOCATION: Isoform 3: Secreted, SUBCELLµLAR LOCATION: Isoform 4: Secreted, SUBCELLµLAR LOCATION: Isoform 5: Secreted, SUBCELLµLAR LOCATION: Isoform 6: Secreted Protein Families: Tissue Specificity:Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6. Paythway:MAPKsignalingpathway HGNC Database Link:https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=HGNC:11920 UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=244139 KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?hsa:355 STRING Database Link:https://string-db.org/network/9606.ENSP00000347979 OMIM Database Link:https://www.omim.org/entry/134637134637134637 Lead Time Guidance:18-23 business days