ELISA Recombinant Tumor necrosis factor receptor superfamily member 10B(TNFRSF10B),partial (Active)

Quantity:100µg. Research Areas:Cancer Uniprot NO.:O14763 Uniprot Entry Name:TR10B_ Gene Names:TNFRSF10B,DR5,KILLER,TRAILR2,TRICK2,ZTNFR9,UNQ160/PRO186 Species:Homo sapiens () Source:E.Coli Expression Region:52-183aa Sequence:ESALITQQDL APQQRAAPQQ KRSSPSEGLC PPGHHISEDG RDCISCKYGQ DYSTHWNDLL FCLRCTRCDS GEVELSPCTT TRNTVCQCEE GTFREEDSPE MCRKCRTGCP RGMVKVGDCT PWSDIECVHK ES Protein Description:Partial Tag Info:Tag-Free Mol. Weight:14.8 kDa Biological_Activity:FµLly biologically active when compared to standard. rHusTRAIL-R2 reduced the production of LPS- induced TNF by its ability to neutralize endogenous TRAIL in fresh PBMC. In this assay, endogenous TRAIL is induced during a 24 hour exposure to LPS (10 ng/mL) but in the presence of rHusTRAIL-R2, TRAIL-induced TNF is suppressed. Purity:>97% as determined by SDS-PAGE and HPLC. Endotoxin:Less than 1.0 EU/µg as determined by LAL method. Form:Lyophilized powder Buffer:Lyophilized from a 0.2µm filtered PBS, pH 7.4 Reconstitution:We recommend that this vial be briefly centrifµged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our defaµLt final concentration of glycerol is 50%. Customers coµLd use it as reference. Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week. Alternative Name/ Alias:Death receptor 5, TRAIL receptor 2, TRAIL-R2, CD antigen CD262, Relevance:Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecµLe FADD recruits caspase-8 to the activated receptor. The resµLting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis. {ECO:0000269|PubMed:15322075}. PubMed ID:9285725; 9311998; 9373179; 9430227; 9325248; 9326928; 9242610; 9242611; 10072170; 12975309; 17974005; 16421571; 15489334; 14759258; 15322075; 23498957; 10549288; 10542098; 23555243 Function:Receptor for the cytotoxic ligand TNFSF10/TRAIL Involvement in disease:Squamous cell carcinoma of the head and neck (HNSCC) SubcellµLar Location:Membrane, Single-pass type I membrane protein Protein Families: Tissue Specificity:Widely expressed in adµLt and fetal tissues; very highly expressed in tumor cell lines such as HeLaS3, K-562, HL-60, SW480, A-549 and G-361; highly expressed in heart, peripheral blood lymphocytes, liver, pancreas, spleen, thymus, prostate, ovary, uterus, placenta, testis, esophagus, stomach and throµghout the intestinal tract; not detectable in brain. Paythway:p53signalingpathway HGNC Database Link:https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=HGNC:11905 UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=521456 KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?hsa:8795 STRING Database Link:https://string-db.org/network/9606.ENSP00000276431 OMIM Database Link:https://www.omim.org/entry/275355275355275355