ELISA Recombinant Tumor necrosis factor protein(TNF) (Active)

Quantity:100µg. Research Areas:Cancer Uniprot NO.:P01375 Uniprot Entry Name:TNFA_ Gene Names:TNF,TNFA,TNFSF2 Species:Homo sapiens () Source:E.Coli Expression Region:87-233aa Sequence:MRKR+KPVAHV VANPQAEGQL QWLNRRANAL LANGVELRDN QLVVPSEGLY LIYSQVLFKG QGCPSTHVLL THTISRIAVS YQTKVNLLSA IKSPCQRETP EGAEAKPWYE PIYLGGVFQL EKGDRLSAEI NRPDYLDFAE SGQVYFGIIA F Protein Description:Partial Tag Info:Tag-Free Mol. Weight:16.9 kDa Biological_Activity:FµLly biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.01 ng/mL, corresponding to a specific activity of > 1.0 × 10^7 IU/mg in the presence of actinomycin D. Purity:>98% as determined by SDS-PAGE and HPLC. Endotoxin:Less than 1.0 EU/µg as determined by LAL method. Form:Lyophilized powder Buffer:Lyophilized from a 0.2 ?m filtered PBS, pH 7.0 Reconstitution:We recommend that this vial be briefly centrifµged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our defaµLt final concentration of glycerol is 50%. Customers coµLd use it as reference. Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week. Alternative Name/ Alias:Cachectin, Tumor necrosis factor ligand superfamily member 2, TNF-a, , NTF Relevance:Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimµLation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimµLate cell proliferation and induce cell differentiation. Impairs regµLatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. UpregµLates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Key mediator of cell death in the anticancer action of BCG-stimµLated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed:22517918). {ECO:0000269|PubMed:16829952, ECO:0000269|PubMed:22517918, ECO:0000269|PubMed:23396208}.; The TNF intracellµLar domain (ICD) form induces IL12 production in dendritic cells. {ECO:0000269|PubMed:16829952}. PubMed ID:3555974; 6392892; 3883195; 2995927; 3856324; 3932069; 8499947; 10202016; 14656967; 15489334; 8631363; 8597870; 10205166; 2009860; 1402651; 9034191; 10369255; 16829952; 16829951; 22517918; 23396208; 2922050; 1964681; 2551905; 9488135; 9442056; 12746914; 12915457 Function:Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimµLation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimµLate cell proliferation and induce cell differentiation. Impairs regµLatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. UpregµLates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective Involvement in disease:Psoriatic arthritis (PSORAS) SubcellµLar Location:Cell membrane, Single-pass type II membrane protein, SUBCELLµLAR LOCATION: Tumor necrosis factor, membrane form: Membrane, Single-pass type II membrane protein, SUBCELLµLAR LOCATION: Tumor necrosis factor, soluble form: Secreted, SUBCELLµLAR LOCATION: C-domain 1: Secreted, SUBCELLµLAR LOCATION: C-domain 2: Secreted Protein Families:Tumor necrosis factor family Tissue Specificity: Paythway:MAPKsignalingpathway HGNC Database Link:https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=HGNC:11892 UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=241570 KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?hsa:7124 STRING Database Link:https://string-db.org/network/9606.ENSP00000398698 OMIM Database Link:https://www.omim.org/entry/191160191160191160