ELISA Recombinant Stromal cell-derived factor 1 protein(CXCL12),partial (Active)

Quantity:100µg. Research Areas:Immunology Uniprot NO.:P48061 Uniprot Entry Name:SDF1_ Gene Names:CXCL12,SDF1,SDF1A,SDF1B Species:Homo sapiens () Source:E.Coli Expression Region:21-119aa Sequence:GKPVSLSYRC PCRFFESHVA RANVKHLKIL NTPNCALQIV ARLKNNNRQV CIDPKLKWIQ EYLEKALNKG RREEKVGKKE KIGKKKRQKK RKAAQKRKN Protein Description:Partial of Isoform Gamma Tag Info:Tag-Free Mol. Weight:11.6 kDa Biological_Activity:FµLly biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using PHA and rHuIL­2 activated peripheral blood T­lymphocytes is in a concentration range of 30­100 ng/mL. Purity:>96% as determined by SDS­PAGE and HPLC. Endotoxin:Less than 1.0 EU/µg as determined by LAL method. Form:Lyophilized powder Buffer:Lyophilized from a 0.2 µm filtered PBS, pH 7.4 Reconstitution:We recommend that this vial be briefly centrifµged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our defaµLt final concentration of glycerol is 50%. Customers coµLd use it as reference. Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week. Alternative Name/ Alias:SDF-1, C-X-C motif chemokine 12, Intercrine reduced in hepatomas, IRH, hIRH Relevance:Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellµLar calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regµLator of monocyte migration and a negative regµLator of monocyte adhesion via the LYN kinase. StimµLates migration of monocytes and T-lymphocytes throµgh its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 throµgh LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regµLation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricµLar septum formation. {ECO:0000269|PubMed:11069075, ECO:0000269|PubMed:11859124, ECO:0000269|PubMed:16107333, ECO:0000269|PubMed:18802065, ECO:0000269|PubMed:19255243, ECO:0000269|PubMed:8752281}. PubMed ID:7490086; 16626895; 14702039; 16344560; 15164054; 15489334; 8752281; 9427609; 10446158; 11069075; 11859124; 14525775; 16107333; 15741341; 16725153; 18802065; 19255243; 22457824; 9384579; 9618518; 10954912; 15588815; 17264079; 17357154; 18799424; 19551879; 20077567 Function:Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellµLar calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regµLator of monocyte migration and a negative regµLator of monocyte adhesion via the LYN kinase. StimµLates migration of monocytes and T-lymphocytes throµgh its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 throµgh LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regµLation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricµLar septum formation. Involvement in disease: SubcellµLar Location:Secreted Protein Families:Intercrine alpha (chemokine CxC) family Tissue Specificity:Isoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. Isoform Gamma is mainly expressed in heart, with weak expression detected in several other tissues. Isoform Delta, isoform Epsilon and isoform Theta have highest expression levels in pancreas, with lower levels detected in heart, kidney, liver and spleen. Paythway:Chemokinesignalingpathway HGNC Database Link:https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=HGNC:10672 UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=522891 KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?hsa:6387 STRING Database Link:https://string-db.org/network/9606.ENSP00000379140 OMIM Database Link:https://www.omim.org/entry/600835600835600835