ELISA 1,25-dihydroxyvitamin D (CYP24A1)

Reactivity: (Homo sapiens) UniProt:Q07973 Abbreviation:CYP24A1 Alternative Names:CP24; CYP24; MGC126273; MGC126274; P450-CC24; 1;25-@dihydroxyvitamin D3 24-hydroxylase|24-OHase|cytochrome P450 family 24 subfamily A polypeptide 1|cytochrome P450; subfamily XXIV (vitamin D 24-hydr Application:ELISA Range:0.31-20 ng/mL Sensitivity:0.156 ng/mL Intra-AssayCV:?3.4% Inter-AssayCV:?8.0% Recovery:0.8 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate CYP24A1 in samples. An antibody specific for CYP24A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCYP24A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for CYP24A1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYP24A1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview: Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).