ELISA 3-hydroxyacyl-CoA dehydratase 1 (PTPLA)

Reactivity: (Homo sapiens) UniProt:B0YJ81 Abbreviation:PTPLA Alternative Names:CAP; cementum attachment protein|protein tyrosine phosphatase-like; member A|protein-tyrosine phosphatase-like member A Application:ELISA Range:0.781-50 ng/mL Sensitivity:0.27 ng/mL Intra-AssayCV:?5.2% Inter-AssayCV:?9.6% Recovery:1.03 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate PTPLA in samples. An antibody specific for PTPLA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTPLA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PTPLA is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTPLA bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:PTPLA contains a characteristic catalytic motif of the protein tyrosine phosphatases (PTPs) family. The PTP motif of this protein has the highly conserved arginine residue replaced by a proline residue; thus it may represent a distinct class of PTPs. Members of the PTP family are known to be signaling molecµLes that regµLate a variety of cellµLar processes. This gene was preferentially expressed in both adµLt and fetal heart. A much lower expression level was detected in skeletal and smooth muscle tissues, and no expression was observed in other tissues. The tissue specific expression in the developing and adµLt heart sµggests a role in regµLating cardiac development and differentiation. Highly expressed in the myocardium, and to a lesser extent in skeletal and smooth muscµLar tissues including those from stomach, jejunum, and bladder. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).