ELISA A disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5)

Reactivity: (Homo sapiens) UniProt:Q9TT92 Abbreviation:ADAMTS5 Alternative Names:ADAMTS11; ADMP-2; FLJ36738; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif; 5 (aggrecanase-2)|aggrecanase-2 Application:ELISA Range:0.312-20 ng/mL Sensitivity:0.112 ng/mL Intra-AssayCV:?4.4% Inter-AssayCV:?8.0% Recovery:1.08 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate ADAMTS5 in samples. An antibody specific for ADAMTS5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADAMTS5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for ADAMTS5 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAMTS5 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:ADAMTS5 is a member of the ADAMTS protein family. Members of the family share several distinct protein modµLes, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene contains two C-terminal TS motifs and functions as aggrecanase to cleave aggrecan, a major proteoglycan of cartilage. Genetically modified mice in which the catalytic domain of ADAMTS5 was deleted are resistant to cartilage destruction in an experimental model of osteoarthritis.ADAMTS5 is the major aggrecanase in mouse cartilage in a mouse model of inflammatory arthritis. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).