ELISA 4F2 cell-surface antigen heavy chain (SLC3A2)

Reactivity: (Homo sapiens) UniProt:P08195 Abbreviation:SLC3A2 Alternative Names:4F2; 4F2HC; 4T2HC; CD98; CD98HC; MDU1; NACAE; 4F2 cell-surface antigen heavy chain|4F2 heavy chain|CD98 heavy chain|antigen defined by monoclonal antibody 4F2; heavy chain|antigen identified by mono Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.061 ng/mL Intra-AssayCV:?4.9% Inter-AssayCV:?6.2% Recovery:0.87 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate SLC3A2 in samples. An antibody specific for SLC3A2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC3A2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for SLC3A2 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC3A2 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:SLC3A2 is a member of the solute carrier family and encodes a cell surface, transmembrane protein with an alpha-amylase domain. The protein exists as the heavy chain of a heterodimer, covalently bound throµgh di-sµLfide bonds to one of several possible light chains. It associates with integrins and mediates integrin-dependent signaling related to normal cell growth and tumorigenesis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.Expressed ubiquitously in all tissues tested with highest levels detected in kidney, placenta and testis and weakest level in thymus. During gestation, expression in the placenta was significantly stronger at fµLl-term than at the mid- trimester stage. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).